growth factor tgf β (Bioss)
Structured Review

Growth Factor Tgf β, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/growth factor tgf β/product/Bioss
Average 95 stars, based on 114 article reviews
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1) Product Images from "Latilactobacillus curvatus DCF0620 and postbiotics derived from soybean germ reduce colitis severity by modulating fibrosis and gut dysbiosis"
Article Title: Latilactobacillus curvatus DCF0620 and postbiotics derived from soybean germ reduce colitis severity by modulating fibrosis and gut dysbiosis
Journal: Frontiers in Immunology
doi: 10.3389/fimmu.2025.1726298
Figure Legend Snippet: L. paracasei DCF0420, L. plantarum DCF0514, and L. curvatus DCF0620 improve inflammation and fibrosis in vitro . (A) Splenocytes from C57BL/6 mice (n = 4-6) were stimulated with each strain separately (at 0.1, 1, or 10 μg/mL) or vehicle (saline) for 2 h and then cultured with anti-CD3 Ab (2 μg/mL) or LPS (500 ng/mL) for 3 days. The levels of IFN-γ, IL-17 and IL-10 in the culture supernatant were determined via ELISA. (B) CCD-18Co cells were treated with each strain separately (at 10 μg/mL) or vehicle (saline) in the presence of TGF-β (10 ng/mL) for 24 h. The levels of fibronectin were assessed via immunoblotting. Values are presented as means ± SDs. Data are representative of two independent experiments.
Techniques Used: In Vitro, Saline, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot
Figure Legend Snippet: L. paracasei DCF0420, L. plantarum DCF0514, and L. curvatus DCF0620 reduce colonic fibrosis in mice with colitis. Acute colitis was induced in C57BL/6 mice by oral administration of 2% DSS. Each strain was orally administered to the mice from 7 days before DSS injection until the end of the experiment (n = 4-5/group, normal control n = 3). On day 11, colon tissue sections were stained with Masson’s trichrome and Abs against TGF-β, Col1, and α-SMA. Representative images are shown (original magnification: 400×, Scale bar: 100 µm). Graphs display the numbers of Ab-positive cells per field. Data are expressed as mean ± SDs. Statistical analysis was performed using One-way ANOVA followed by Tukey’s test. *P < 0.05, ****P < 0.0001.
Techniques Used: Injection, Control, Staining
Figure Legend Snippet: Postbiotics from L. curvatus DCF0620 reduce colonic fibrosis and inflammation in mice with colitis. Acute colitis was induced in C57BL/6 mice by oral administration of 2% DSS. Then, 200 μL of postbiotics (100 mg/mL in PBS) was orally administered to the mice from 7 days before DSS injection until the end of the experiment (n = 4-5/group, normal control n = 3). (A) On day 12, colon tissue sections were stained with Abs against TGF-β, Col1, and α-SMA. (B) On day 12, the sections were stained with Abs against TNF-α, IL-1β, IL-6, and IL-17. Representative images are shown (original magnification: 400×, Scale bar: 100 µm). Graphs display the numbers of Ab-positive cells per field. Data are expressed as mean ± SDs. Statistical analysis was performed using One-way ANOVA followed by Tukey’s test. ****P < 0.0001.
Techniques Used: Injection, Control, Staining
